b2m based stimulation Search Results


99
Revvity il 6
Macrophage marker, inflammatory <t>cytokine</t> and MMP mRNA levels in synovial macrophages following exposure to β2-microglobulin (B2M). mRNA expression of A ) CD80 , B ) CD163 , C ) CD206 , D <t>)</t> <t>IL-6</t> , E ) IL-8 , F ) TNFA . * p < 0.05 G ) MMP-9 and H ) MMP-13 in synovial macrophages after stimulation with B2M for 6 and 24 h. * p < 0.05
Il 6, supplied by Revvity, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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95
Miltenyi Biotec b2m based stimulation
Macrophage marker, inflammatory <t>cytokine</t> and MMP mRNA levels in synovial macrophages following exposure to β2-microglobulin (B2M). mRNA expression of A ) CD80 , B ) CD163 , C ) CD206 , D <t>)</t> <t>IL-6</t> , E ) IL-8 , F ) TNFA . * p < 0.05 G ) MMP-9 and H ) MMP-13 in synovial macrophages after stimulation with B2M for 6 and 24 h. * p < 0.05
B2m Based Stimulation, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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99
Thermo Fisher gene exp axin2 mm00443610 m1
Macrophage marker, inflammatory <t>cytokine</t> and MMP mRNA levels in synovial macrophages following exposure to β2-microglobulin (B2M). mRNA expression of A ) CD80 , B ) CD163 , C ) CD206 , D <t>)</t> <t>IL-6</t> , E ) IL-8 , F ) TNFA . * p < 0.05 G ) MMP-9 and H ) MMP-13 in synovial macrophages after stimulation with B2M for 6 and 24 h. * p < 0.05
Gene Exp Axin2 Mm00443610 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Miltenyi Biotec pan t cell isolation kit
Macrophage marker, inflammatory <t>cytokine</t> and MMP mRNA levels in synovial macrophages following exposure to β2-microglobulin (B2M). mRNA expression of A ) CD80 , B ) CD163 , C ) CD206 , D <t>)</t> <t>IL-6</t> , E ) IL-8 , F ) TNFA . * p < 0.05 G ) MMP-9 and H ) MMP-13 in synovial macrophages after stimulation with B2M for 6 and 24 h. * p < 0.05
Pan T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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99
DiaSorin Biotechnology 200 system
Macrophage marker, inflammatory <t>cytokine</t> and MMP mRNA levels in synovial macrophages following exposure to β2-microglobulin (B2M). mRNA expression of A ) CD80 , B ) CD163 , C ) CD206 , D <t>)</t> <t>IL-6</t> , E ) IL-8 , F ) TNFA . * p < 0.05 G ) MMP-9 and H ) MMP-13 in synovial macrophages after stimulation with B2M for 6 and 24 h. * p < 0.05
200 System, supplied by DiaSorin Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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90
Myelo Therapeutics GmbH myelo peroxidase (mpo)
Coefficients of variation based on 84 replicates and Pearson correlations (in parentheses) based on 40 paired serial samples for assays in the common phase II set by site. *
Myelo Peroxidase (Mpo), supplied by Myelo Therapeutics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Macrophage marker, inflammatory cytokine and MMP mRNA levels in synovial macrophages following exposure to β2-microglobulin (B2M). mRNA expression of A ) CD80 , B ) CD163 , C ) CD206 , D ) IL-6 , E ) IL-8 , F ) TNFA . * p < 0.05 G ) MMP-9 and H ) MMP-13 in synovial macrophages after stimulation with B2M for 6 and 24 h. * p < 0.05

Journal: Central-European Journal of Immunology

Article Title: β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium

doi: 10.5114/ceji.2022.124092

Figure Lengend Snippet: Macrophage marker, inflammatory cytokine and MMP mRNA levels in synovial macrophages following exposure to β2-microglobulin (B2M). mRNA expression of A ) CD80 , B ) CD163 , C ) CD206 , D ) IL-6 , E ) IL-8 , F ) TNFA . * p < 0.05 G ) MMP-9 and H ) MMP-13 in synovial macrophages after stimulation with B2M for 6 and 24 h. * p < 0.05

Article Snippet: To analyze the effect of B2M on IL-6, IL-8, TNF-α and MMP-13 production, we conducted enzyme-linked immunosorbent assay (ELISA) on the culture supernatant using a commercially available kit (LEGEND MAX Human ELISA Kit, BioLegend, San Diego, CA, USA) based on the manufacturer’s protocol.

Techniques: Marker, Expressing

Amounts of IL-6, IL-8, TNF-α and MMP-13 generated by synovial macrophages following exposure to β2-microglobulin (B2M) determined using ELISA. Protein concentrations of A ) IL6, B ) IL8, C ) TNF-α and D ) MMP-13 produced by synovial macrophages after stimulation with B2M for 6 or 24 h. * p < 0.05

Journal: Central-European Journal of Immunology

Article Title: β2-microglobulin alters the profiles of inflammatory cytokines and of matrix metalloprotease in macrophages derived from the osteoarthritic synovium

doi: 10.5114/ceji.2022.124092

Figure Lengend Snippet: Amounts of IL-6, IL-8, TNF-α and MMP-13 generated by synovial macrophages following exposure to β2-microglobulin (B2M) determined using ELISA. Protein concentrations of A ) IL6, B ) IL8, C ) TNF-α and D ) MMP-13 produced by synovial macrophages after stimulation with B2M for 6 or 24 h. * p < 0.05

Article Snippet: To analyze the effect of B2M on IL-6, IL-8, TNF-α and MMP-13 production, we conducted enzyme-linked immunosorbent assay (ELISA) on the culture supernatant using a commercially available kit (LEGEND MAX Human ELISA Kit, BioLegend, San Diego, CA, USA) based on the manufacturer’s protocol.

Techniques: Generated, Enzyme-linked Immunosorbent Assay, Produced

Coefficients of variation based on 84 replicates and Pearson correlations (in parentheses) based on 40 paired serial samples for assays in the common phase II set by site. *

Journal:

Article Title: Ovarian Cancer Biomarker Performance in Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial Specimens

doi: 10.1158/1940-6207.CAPR-10-0195

Figure Lengend Snippet: Coefficients of variation based on 84 replicates and Pearson correlations (in parentheses) based on 40 paired serial samples for assays in the common phase II set by site. *

Article Snippet: The least stable markers were prolactin, cytokeratin 19, growth hormone, myeloperoxidase, tumor necrosis factor receptor 2, and kallikrein 6. table ft1 table-wrap mode="anchored" t5 caption a7 Marker Name Partners CV (r) FHCRC CV (r) MDA CV (r) UPC1 CV (r) Alpha-fetoprotein (AFP) 0.15 (0.92) Apolipoprotein A1 (APO) 0.23 (0.39) B7-H4 0.29 (0.63) Beta 2 – Microglobulin (B2M) 0.10 (0.64) CA125 0.10 (0.94) 0.12 (0.92) 0.61 (0.80) CA15.3 0.12 (0.89) 0.26 (0.89) CA19.9 0.09 (0.89) 0.30 (0.70) CA72.4 0.19 (0.69) 0.14 (0.88) Carcinogenic embryonic antigen (CEA) 0.11 (0.83) Chitinase 0.17 (0.44) Connective tissue activating protein III (CTAP-III) 0.12 (0.62) Cytokeratin 19 0.33 (0.01) Decoy Receptor 3 (DcR3) 0.06 (0.64) Epidermanl Growth Factor Receptor (EGFR) 0.13 (0.83) EOTAXIN 0.52 (0.34) ErbB2 0.14 (0.76) Follicle Stimulating Hormone (FSH) 0.18 (0.81) Growth hormone (GH) 0.29 (0.17) Human epididymis protein 4 (HE4) 0.06 (0.74) 0.25 (0.78) 0.23 (0.87) Hepcidin (HEPC) 0.18 (0.56) Insulin-like growth factor 2 (IGF2) 0.07 (0.72) Insulin-like growth factor binding protein 1 (IGFBP1) 0.25 (0.61) Insulin-like growth factor binding protein 2 (IGFBP2) 0.16 (0.82) Interleukin 10 (IL10) 0.27 (0.64) Interleukin 2 (IL2R) 0.28 (0.55) Interleukin 6 (IL6) 0.28 (0.72) Interleukin 6 Receptor (IL6R) 0.15 (0.84) Interleukin 8 (IL8) 0.32 (0.52) Inter-alpha-trypsin inhibitor heavy chain (ITIH4) 0.21 (0.59) Kallikrein 6 (KLK6) 0.21 (0.32) Kallikrein 8 0.32 (0.52) Luteinizing Hormone (LH) 0.19 (0.84) Macrophage inhibiting factor (MIF) 0.41 (0.52) Macrophage inhibitory cytokine 1 0.41 (0.83) Mesothelin 0.42 (0.72) 0.16 (0.75) 0.18 (0.88) Metallomatrix Protein 2 (MMP2) 0.14 (0.42) Metallomatrix Protein 3 (MMP3) 0.15 (0.74) Metallomatrix Protein 9 (MMP9) 0.19 (0.33) Myelo peroxidase (MPO) 0.44 (0.23) Prolactin 0.24 (0.01) Soluble intracellular adhesion molecule 1 0.31 (0.43) Soluble vascular cell adhesion molecule 1 (sV-CAM) 0.25 (0.54) Spondin-2 0.13 (0.64) 0.17 (0.63) Tumor Necrosis Factor Alpha (TNFa) 0.27 (0.38) Tumor Necrosis Factor Receptor 2 (TNFr2) 0.50 (0.28) Total Plasminogen Activator Inhibitor (tPAI1) 0.32 (0.54) Transthyretin 0.18 (0.39) Transferrin (TRF) 0.17 (0.38) Thyroid Stimulating Hormone (TSH) 0.21 (0.77) Open in a separate window * Partners performed platform (Roche E170) or plate-based assays; FHCRC performed single-plex Luminex bead assays and plate-based assays; MDACC performed a mass spectographic analysis using surface-enhanced antibody chips; UPCI performed multiplexed Luminex bead assays.

Techniques: Marker, Binding Assay

Phase II results for all cases and early stage cases compared to general population controls by sensitivity at 95% specificity and area under the curve (sorted by sensitivity for all cases).

Journal:

Article Title: Ovarian Cancer Biomarker Performance in Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial Specimens

doi: 10.1158/1940-6207.CAPR-10-0195

Figure Lengend Snippet: Phase II results for all cases and early stage cases compared to general population controls by sensitivity at 95% specificity and area under the curve (sorted by sensitivity for all cases).

Article Snippet: The least stable markers were prolactin, cytokeratin 19, growth hormone, myeloperoxidase, tumor necrosis factor receptor 2, and kallikrein 6. table ft1 table-wrap mode="anchored" t5 caption a7 Marker Name Partners CV (r) FHCRC CV (r) MDA CV (r) UPC1 CV (r) Alpha-fetoprotein (AFP) 0.15 (0.92) Apolipoprotein A1 (APO) 0.23 (0.39) B7-H4 0.29 (0.63) Beta 2 – Microglobulin (B2M) 0.10 (0.64) CA125 0.10 (0.94) 0.12 (0.92) 0.61 (0.80) CA15.3 0.12 (0.89) 0.26 (0.89) CA19.9 0.09 (0.89) 0.30 (0.70) CA72.4 0.19 (0.69) 0.14 (0.88) Carcinogenic embryonic antigen (CEA) 0.11 (0.83) Chitinase 0.17 (0.44) Connective tissue activating protein III (CTAP-III) 0.12 (0.62) Cytokeratin 19 0.33 (0.01) Decoy Receptor 3 (DcR3) 0.06 (0.64) Epidermanl Growth Factor Receptor (EGFR) 0.13 (0.83) EOTAXIN 0.52 (0.34) ErbB2 0.14 (0.76) Follicle Stimulating Hormone (FSH) 0.18 (0.81) Growth hormone (GH) 0.29 (0.17) Human epididymis protein 4 (HE4) 0.06 (0.74) 0.25 (0.78) 0.23 (0.87) Hepcidin (HEPC) 0.18 (0.56) Insulin-like growth factor 2 (IGF2) 0.07 (0.72) Insulin-like growth factor binding protein 1 (IGFBP1) 0.25 (0.61) Insulin-like growth factor binding protein 2 (IGFBP2) 0.16 (0.82) Interleukin 10 (IL10) 0.27 (0.64) Interleukin 2 (IL2R) 0.28 (0.55) Interleukin 6 (IL6) 0.28 (0.72) Interleukin 6 Receptor (IL6R) 0.15 (0.84) Interleukin 8 (IL8) 0.32 (0.52) Inter-alpha-trypsin inhibitor heavy chain (ITIH4) 0.21 (0.59) Kallikrein 6 (KLK6) 0.21 (0.32) Kallikrein 8 0.32 (0.52) Luteinizing Hormone (LH) 0.19 (0.84) Macrophage inhibiting factor (MIF) 0.41 (0.52) Macrophage inhibitory cytokine 1 0.41 (0.83) Mesothelin 0.42 (0.72) 0.16 (0.75) 0.18 (0.88) Metallomatrix Protein 2 (MMP2) 0.14 (0.42) Metallomatrix Protein 3 (MMP3) 0.15 (0.74) Metallomatrix Protein 9 (MMP9) 0.19 (0.33) Myelo peroxidase (MPO) 0.44 (0.23) Prolactin 0.24 (0.01) Soluble intracellular adhesion molecule 1 0.31 (0.43) Soluble vascular cell adhesion molecule 1 (sV-CAM) 0.25 (0.54) Spondin-2 0.13 (0.64) 0.17 (0.63) Tumor Necrosis Factor Alpha (TNFa) 0.27 (0.38) Tumor Necrosis Factor Receptor 2 (TNFr2) 0.50 (0.28) Total Plasminogen Activator Inhibitor (tPAI1) 0.32 (0.54) Transthyretin 0.18 (0.39) Transferrin (TRF) 0.17 (0.38) Thyroid Stimulating Hormone (TSH) 0.21 (0.77) Open in a separate window * Partners performed platform (Roche E170) or plate-based assays; FHCRC performed single-plex Luminex bead assays and plate-based assays; MDACC performed a mass spectographic analysis using surface-enhanced antibody chips; UPCI performed multiplexed Luminex bead assays.

Techniques: